A nascent proteome study combining click chemistry with 2DE

  title={A nascent proteome study combining click chemistry with 2DE},
  author={Ilya A. Osterman and Alexey V. Ustinov and D. V. Evdokimov and Vladimir A. Korshun and Petr V. Sergiev and Marina V. Serebryakova and I. A. Demina and Maria A. Galyamina and Vadim M. Govorun and Olga A. Dontsova},
To investigate the dynamic cellular response to a condition change, selective labeling of the nascent proteome is necessary. Here, we report a method combining click chemistry protein labeling with 2D DIGE. To test the relevance of the method, we compared nascent proteomes of actively growing bacterial cells with that of cells exposed to protein synthesis inhibitor, erythromycin. Cells were incubated with methionine analog, homopropargyl glycin, and their nascent proteome was selectively… 

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