A nanoporous reactor for efficient proteolysis.

  title={A nanoporous reactor for efficient proteolysis.},
  author={Liang Qiao and Yun Liu and Sarah P. Hudson and Pengyuan Y. Yang and Edmond Magner and Baohong Liu},
  volume={14 1},
A nanoreactor based on mesoporous silicates is described for efficient tryptic digestion of proteins within the mesochannels. Cyano-functionalized mesoporous silicate (CNS), with an average pore diameter of 18 nm, is a good support for trypsin, with rapid in situ digestion of the model proteins, cytochrome c and myoglobin. The generated peptides were analyzed by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS). Proteolysis by trypsin-CNS is much more… CONTINUE READING
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