A mutational analysis of the globotriaosylceramide-binding sites of verotoxin VT1.

@article{Soltyk2002AMA,
  title={A mutational analysis of the globotriaosylceramide-binding sites of verotoxin VT1.},
  author={Anna M Soltyk and Colin R MacKenzie and Vince M Wolski and Tomoko Hirama and Pavel I. Kitov and David R Bundle and James L. Brunton},
  journal={The Journal of biological chemistry},
  year={2002},
  volume={277 7},
  pages={5351-9}
}
Escherichia coli verotoxin, also known as Shiga-like toxin, binds to eukaryotic cell membranes via the glycolipid Gb(3) receptors which present the P(k) trisaccharide Galalpha(1-4)Galbeta(1-4)Glcbeta. Crystallographic studies have identified three P(k) trisaccharide (P(k)-glycoside) binding sites per verotoxin 1B subunit (VT1B) monomer while NMR studies have identified binding of P(k)-glycoside only at site 2. To understand the basis for this difference, we studied binding of wild type VT1B and… CONTINUE READING

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