A mutation that alters the nucleotide specificity of elongation factor Tu, a GTP regulatory protein.

@article{Hwang1987AMT,
  title={A mutation that alters the nucleotide specificity of elongation factor Tu, a GTP regulatory protein.},
  author={Yu Wen Hwang and Daniel L Miller},
  journal={The Journal of biological chemistry},
  year={1987},
  volume={262 27},
  pages={13081-5}
}
A single amino acid substitution (Asp to Asn) at position 138 of Escherichia coli elongation factor Tu (EF-Tu) was introduced in the tufA gene clone by oligonucleotide site-directed mutagenesis. The mutated tufA gene was then expressed in maxicells. The properties of [35S]methionine-labeled mutant and wild type EF-Tu were compared by in vitro assays. The Asn-138 mutation greatly reduced the protein's affinity for GDP; however, this mutation dramatically increased the protein's affinity for… CONTINUE READING