A mutant of Escherichia coli defective in the first step of endotoxin biosynthesis.

@article{Galloway1990AMO,
  title={A mutant of Escherichia coli defective in the first step of endotoxin biosynthesis.},
  author={Sue M. Galloway and Christian R. H. Raetz},
  journal={The Journal of biological chemistry},
  year={1990},
  volume={265 11},
  pages={6394-402}
}
Using localized mutagenesis of whole cells, we have isolated a temperature-sensitive UDP-N-acetylglucosamine acyltransferase mutant of Escherichia coli that loses all detectable acyltransferase activity and quickly dies after a shift from 30 to 42 degrees C. Acyltransferase activity and temperature resistance are restored by transforming the mutant with a hybrid plasmid containing the E. coli gene for UDP-GlcNAc acyltransferase (lpxA). In addition, a new assay has been developed for… CONTINUE READING
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