A mutant herpes simplex virus type 1 thymidine kinase reporter gene shows improved sensitivity for imaging reporter gene expression with positron emission tomography.

@article{Gambhir2000AMH,
  title={A mutant herpes simplex virus type 1 thymidine kinase reporter gene shows improved sensitivity for imaging reporter gene expression with positron emission tomography.},
  author={S. Gambhir and E. Bauer and M. Black and Q. Liang and M. S. Kokoris and J. Barrio and Meera R. Iyer and M. Namavari and M. Phelps and H. Herschman},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2000},
  volume={97 6},
  pages={
          2785-90
        }
}
  • S. Gambhir, E. Bauer, +7 authors H. Herschman
  • Published 2000
  • Medicine, Biology
  • Proceedings of the National Academy of Sciences of the United States of America
  • We are developing assays for noninvasive, quantitative imaging of reporter genes with positron emission tomography (PET), for application both in animal models and in human gene therapy. We report here a method to improve the detection of lower levels of PET reporter gene expression by utilizing a mutant herpes simplex virus type 1 thymidine kinase (HSV1-sr39tk) as a PET reporter gene. The HSV1-sr39tk mutant was identified from a library of site-directed mutants. Accumulation (net uptake) of… CONTINUE READING

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