A mutant herpes simplex virus type 1 thymidine kinase reporter gene shows improved sensitivity for imaging reporter gene expression with positron emission tomography.

@article{Gambhir2000AMH,
  title={A mutant herpes simplex virus type 1 thymidine kinase reporter gene shows improved sensitivity for imaging reporter gene expression with positron emission tomography.},
  author={Sanjiv Sam Gambhir and Eileen M. Bauer and Margaret E. Black and Qianwa Liang and Mark S. Kokoris and Jorge R. Barrio and Meera Iyer and Mohammad Namavari and Michael E. Phelps and Harvey R. Herschman},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2000},
  volume={97 6},
  pages={2785-90}
}
We are developing assays for noninvasive, quantitative imaging of reporter genes with positron emission tomography (PET), for application both in animal models and in human gene therapy. We report here a method to improve the detection of lower levels of PET reporter gene expression by utilizing a mutant herpes simplex virus type 1 thymidine kinase (HSV1-sr39tk) as a PET reporter gene. The HSV1-sr39tk mutant was identified from a library of site-directed mutants. Accumulation (net uptake) of… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 156 extracted citations

Comparison of radiolabeled nucleoside probes (FIAU, FHBG, and FHPG) for PET imaging of HSV1-tk gene expression.

Journal of nuclear medicine : official publication, Society of Nuclear Medicine • 2002
View 9 Excerpts
Highly Influenced

In Vivo Noninvasive Imaging for Gene Therapy

Journal of biomedicine & biotechnology • 2003
View 11 Excerpts
Highly Influenced

Similar Papers

Loading similar papers…