A mutant allele of essential, general translation initiation factor DED1 selectively inhibits translation of a viral mRNA.

@article{Noueiry2000AMA,
  title={A mutant allele of essential, general translation initiation factor DED1 selectively inhibits translation of a viral mRNA.},
  author={Amine O. Noueiry and Jianbo Chen and Paul Ahlquist},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={2000},
  volume={97 24},
  pages={12985-90}
}
Positive-strand RNA virus genomes are substrates for translation, RNA replication, and encapsidation. To identify host factors involved in these functions, we used the ability of brome mosaic virus (BMV) RNA to replicate in yeast. We report herein identification of a mutation in the essential yeast gene DED1 that inhibited BMV RNA replication but not yeast growth. DED1 encodes a DEAD (Asp-Glu-Ala-Asp)-box RNA helicase required for translation initiation of all yeast mRNAs. Inhibition of BMV RNA… CONTINUE READING