A mutant T4 lysozyme (Val 131----Ala) designed to increase thermostability by the reduction of strain within an alpha-helix.

@article{Daopin1990AMT,
  title={A mutant T4 lysozyme (Val 131----Ala) designed to increase thermostability by the reduction of strain within an alpha-helix.},
  author={Sun Dao-pin and W. A. Baase and Brian W. Matthews},
  journal={Proteins},
  year={1990},
  volume={7 2},
  pages={198-204}
}
An attempt has been made to identify residues in T4 phage lysozyme that may have strained conformations and, by appropriate site-directed replacements, to reduce this strain and thus increase the thermostability of the protein. Valine 131, within alpha-helix 126-134, was identified as a potential candidate. Its side-chain rotational angle, chi 1, differs by… CONTINUE READING