A multipurpose transposon system for analyzing protein production, localization, and function in Saccharomyces cerevisiae.

@article{RossMacdonald1997AMT,
  title={A multipurpose transposon system for analyzing protein production, localization, and function in Saccharomyces cerevisiae.},
  author={P B Ross-Macdonald and Amy E. Sheehan and G. Shirleen Roeder and Michael Snyder},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1997},
  volume={94 1},
  pages={190-5}
}
Analysis of the function of a particular gene product typically involves determining the expression profile of the gene, the subcellular location of the protein, and the phenotype of a null strain lacking the protein. Conditional alleles of the gene are often created as an additional tool. We have developed a multifunctional, transposon-based system that simultaneously generates constructs for all the above analyses and is suitable for mutagenesis of any given Saccharomyces cerevisiae gene… CONTINUE READING

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