A multiplex TaqMan real-time PCR for detection and differentiation of four antigenic types of canine parvovirus in China

@article{Sun2018AMT,
  title={A multiplex TaqMan real-time PCR for detection and differentiation of four antigenic types of canine parvovirus in China},
  author={Yaru Sun and Y. Cheng and P. Lin and L. Yi and M. Tong and Z. Cao and G. Wang and S. Li and Shipeng Cheng and W. Yuan and Jianke Wang},
  journal={Molecular and Cellular Probes},
  year={2018},
  volume={38},
  pages={7 - 12}
}
Abstract Canine parvovirus (CPV) is an important pathogen in domestic dogs, and the original antigenic types CPV-2 and its variants, CPV-2a, 2b and 2c, are prevalent worldwide. A multiplex TaqMan real-time PCR method was developed for the detection and differentiation of four antigenic types of CPV. A set of primers and probes, CPV-305F/CPV-305R and CPV-2-305P (for CPV-2)/CPV-2a-305P (for CPV-2a, 2b and 2c), was able to differentiate CPV-2 and its variants (CPV-2a, 2b and 2c). Another set… Expand
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