A multiple fission-like mode of asexual reproduction inBlastocystis hominis

@article{Suresh2004AMF,
  title={A multiple fission-like mode of asexual reproduction inBlastocystis hominis},
  author={Kuru Suresh and Josephine Howe and G. C. Ng and L. C. Ho and N. P. Ramachandran and A. K. Loh and E. H. Yap and M. Singh},
  journal={Parasitology Research},
  year={2004},
  volume={80},
  pages={523-527}
}
A non-axenic and an axenic isolate ofBlastocystis hominis have been induced to form cysts in vitro using an encystation medium. The morphology of the parasite at different time points was observed by scanning electron microscopy. In day-2 cultures the cysts were spherical and had a non-uniform, coarse outer surface around the body. A deep, pore-like opening was seen in some of the parasites. Most of the cysts from day-4 and day-6 cultures ruptured, revealing small, uniformly sized spherical… Expand
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References

SHOWING 1-10 OF 22 REFERENCES
Ultrastructural changes during in vitro encystment ofBlastocystis hominis
TLDR
The morphological changes occurring in Blastocystis hominis at different time points following in vitro encystment were studied by electron microscopy and the amoebic form appeared to be an intermediate stage between the vacuolar form and the pre-cystic form. Expand
Studies of Blastocystis hominis.
  • C. Zierdt
  • Biology, Medicine
  • The Journal of protozoology
  • 1973
SYNOPSIS Blastocystis hominis, grown in Boeck-Drbohlav culture medium, modified by the omission of rice starch and the addition of 20% human serum and mineral oil cover to the Locke's solutionExpand
Ultrastructure of blastocystis hominis
TLDR
Ultrastructural studies of modified Boeck-Drbohlav egg slant cultured Blastocystis hominis reveal ameba, granular, and vacuolated forms of this organism and the usual division by binary fission may be concurrent with central body maturation. Expand
Blastocystis in humans and animals: morphology, biology, and epizootiology.
Publisher Summary This chapter reviews the biology and epizootiology of Blastocystis. The various experimental attempts to induce different forms of Blastocystis hominis (B. hominis) in culture areExpand
Ultrastructural variation of Blastocystis hominis stocks in culture.
TLDR
An ultrastructural study of 10 different Blastocystis hominis stocks was undertaken, and indications of phagocytic behaviour of B. hominis were seen in the amoeboid form and in the vacuolar form of one stock. Expand
Differentiation of Entamoeba: encystation of E. invadens in monoxenic and axenic cultures.
TLDR
A method to stimulate mass encystation of Entamoeba invadens in monoxenic or axenic cultures, using a simple monophasic medium, has been developed and results are described here. Expand
Differentiation of the various stages of Blastocystis hominis by acridine orange staining.
Acridine orange staining differentiates the cystic and the central body forms of Blastocystis hominis and offers a very convenient and easy method to observe the internal structure of the parasite.Expand
The morphology, formation and development of cysts of Entamoeba.
TLDR
The cyst is an integral and critical phase in the life-cycle of Entamoeba; its attributes directly influence the dissemination of the organism, and are an important aspect in the epidemiology of amoebiasis. Expand
In vitro encystment and experimental infections ofBlastocystis hominis
TLDR
Direct stool examination of juvenile Wistar rats infected with 10000 cyst-like stages of both C and MS isolates showed Blastocystis at day 2 post-infection, indicating the importance of the encysted stages in the transmission of the parasite. Expand
Generation time and growth rate of the human intestinal parasite Blastocystis hominis.
TLDR
The generation time (GT) was determined for the 24-hr period during which the most rapid growth occurred; about 2% of the B. hominis cells were in division during this time. Expand
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