A multiple SYBR Green I-based real-time PCR system for the simultaneous detection of porcine circovirus type 2, porcine parvovirus, pseudorabies virus and Torque teno sus virus 1 and 2 in pigs.

@article{Prez2012AMS,
  title={A multiple SYBR Green I-based real-time PCR system for the simultaneous detection of porcine circovirus type 2, porcine parvovirus, pseudorabies virus and Torque teno sus virus 1 and 2 in pigs.},
  author={Lester J P{\'e}rez and Carmen Laura Perera and Mar{\'i}a Teresa Fr{\'i}as and Jos{\'e} I N{\'u}{\~n}ez and Llilianne Ganges and Heidy D{\'i}az de Arce},
  journal={Journal of virological methods},
  year={2012},
  volume={179 1},
  pages={233-41}
}
Multiple viral infections are common in pigs under intensive production conditions. All five of the viruses included in this study are associated with multifactorial diseases that cause significant economic losses in swine farming worldwide. The development is described of a novel multiple real-time PCR system based on the use of SYBR Green I that allows the simultaneous detection and differentiation of porcine circovirus 2 (PCV-2), porcine parvovirus (PPV), pseudorabies virus (PRV) and Torque… CONTINUE READING

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