A multi-step strategy to obtain crystals of the dengue virus RNA-dependent RNA polymerase that diffract to high resolution.

Abstract

Dengue virus, a member of the Flaviviridae genus, causes dengue fever, an important emerging disease with several million infections occurring annually for which no effective therapy exists. The viral RNA-dependent RNA polymerase NS5 plays an important role in virus replication and represents an interesting target for the development of specific antiviral compounds. Crystals that diffract to 1.85 A resolution that are suitable for three-dimensional structure determination and thus for a structure-based drug-design program have been obtained using a strategy that included expression screening of naturally occurring serotype variants of the protein, the addition of divalent metal ions and crystal dehydration.

Cite this paper

@article{Yap2007AMS, title={A multi-step strategy to obtain crystals of the dengue virus RNA-dependent RNA polymerase that diffract to high resolution.}, author={Thai Leong Yap and Yen Liang Chen and Ting Xu and Daying Wen and Subhash G. Vasudevan and Julien Lescar}, journal={Acta crystallographica. Section F, Structural biology and crystallization communications}, year={2007}, volume={63 Pt 2}, pages={78-83} }