A multi-enzyme cascade of hemoglobin proteolysis in the intestine of blood-feeding hookworms.

@article{Williamson2004AMC,
  title={A multi-enzyme cascade of hemoglobin proteolysis in the intestine of blood-feeding hookworms.},
  author={Angela L. Williamson and Paolo Lecchi and Benjamin E. Turk and Youngchool Choe and Peter J Hotez and James H McKerrow and Lewis C. Cantley and Mohammed Sajid and Charles S. Craik and Alex Loukas},
  journal={The Journal of biological chemistry},
  year={2004},
  volume={279 34},
  pages={35950-7}
}
Blood-feeding pathogens digest hemoglobin (Hb) as a source of nutrition, but little is known about this process in multicellular parasites. The intestinal brush border membrane of the canine hookworm, Ancylostoma caninum, contains aspartic proteases (APR-1), cysteine proteases (CP-2), and metalloproteases (MEP-1), the first of which is known to digest Hb. We now show that Hb is degraded by a multi-enzyme, synergistic cascade of proteolysis. Recombinant APR-1 and CP-2, but not MEP-1, digested… CONTINUE READING