A mucus-secreting human colonic epithelial cell line responsive to cholinergic stimulation.

@article{Roumagnac1987AMH,
  title={A mucus-secreting human colonic epithelial cell line responsive to cholinergic stimulation.},
  author={Isabelle Roumagnac and Christian L. Laboisse},
  journal={Biology of the cell},
  year={1987},
  volume={61 1-2},
  pages={65-8}
}
The human colonic epithelial cell line Cl.16E grows in culture as a polarized monolayer which differentiates at confluency into typical goblet cells secreting their mucin content into the culture medium. Polyclonal antibodies raised against these mucins were used in an ELISA to measure the amount of mucins secreted by the Cl.16E cells. Carbachol caused a transient and significant increase in mucus secretion with a maximal stimulation occurring at 30 min. A dose-dependent effect was found with a… CONTINUE READING

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