A mouse monoclonal IgE antibody anti bovine milk beta-lactoglobulin allows studies of allergy in the gastrointestinal tract.

@article{Granato1986AMM,
  title={A mouse monoclonal IgE antibody anti bovine milk beta-lactoglobulin allows studies of allergy in the gastrointestinal tract.},
  author={Dominique Granato and Pierre François Piguet},
  journal={Clinical and experimental immunology},
  year={1986},
  volume={63 3},
  pages={
          703-10
        }
}
A monoclonal IgE antibody directed against bovine milk beta-lactoglobulin (beta-LG) was produced by fusion of NSI myeloma cells with spleen cells of Balb/c mice immunized with alum-precipitated beta-LG. This antibody was found by radioimmunoassay to react with both native and aggregated beta-LG, but a positive passive cutaneous anaphylaxis reaction (PCA) was evident only with aggregated beta-LG. 1 ng of this purified antibody was capable of eliciting a PCA. The chemical and physical properties… CONTINUE READING