A mouse model of intracerebral hemorrhage using autologous blood infusion

@article{Rynkowski2008AMM,
  title={A mouse model of intracerebral hemorrhage using autologous blood infusion},
  author={Michal A Rynkowski and Grace H. Kim and Ricardo J. Komotar and Marc L. Otten and Andrew F. Ducruet and Brad E. Zacharia and Christopher Paul Kellner and David K. Hahn and Maxwell B. Merkow and Matthew C Garrett and Robert M Starke and Byung-Moon Cho and Sergei A. Sosunov and E. Sander Connolly},
  journal={Nature Protocols},
  year={2008},
  volume={3},
  pages={122-128}
}
The development of controllable and reproducible animal models of intracerebral hemorrhage (ICH) is essential for the systematic study of the pathophysiology and treatment of hemorrhagic stroke. In recent years, we have used a modified version of a murine ICH model to inject blood into mouse basal ganglia. According to our protocol, autologous blood is stereotactically infused in two stages into the right striatum to mimic the natural events of hemorrhagic stroke. Following ICH induction… Expand
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TLDR
This double blood infusion model that does not use anticoagulant improves upon the procedure and provides an easy and reproducible alternative for inducing ICH in mice; it should be useful for studying the pathophysiology of ICH and for testing potential pharmaceutical and surgical interventions. Expand
Profiling of Blood-Brain Barrier Disruption in Mouse Intracerebral Hemorrhage Models: Collagenase Injection vs. Autologous Arterial Whole Blood Infusion
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  • Frontiers in Cellular Neuroscience
  • 2021
TLDR
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Three mouse hemorrhagic models that utilize stereotactic injections of autologous whole blood or collagenase to produce ventricular, cortical, and hippocampal injury and reproducible brain damage, brain edema, inflammation, and consistent locomotor deficits are established. Expand
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TLDR
Induction of ICH using both models, immediate post-operative demonstration of injury, and early post-operative care techniques are demonstrated, and both models result in reproducible injuries, hematoma volumes, and neurobehavioral deficits. Expand
History of preclinical models of intracerebral hemorrhage.
TLDR
This review of the various injection models and animal subjects that have been used to study the ICH process while comparing and analyzing the benefits and disadvantages of each is explored. Expand
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TLDR
This review examines the testing of candidate therapeutics in mouse models of ICH for their effect on tissue protection and repair and will help the readers compare it to the extensively researched rat model and thus enhance work that are pending in mouse model. Expand
Neuroprotective effect of neuroserpin in non-tPA-induced intracerebral hemorrhage mouse models
TLDR
The results supported the fact that NSP may be considered as a potential therapy for ICH for the neuroprotective effects including amelioration of the edema, and indicated that protecting and/or repairing the injured vascular endothelial cells may be a potential mechanism involved in NSP neuroprotection. Expand
Rat Brainstem Hemorrhage Model: Key Points to Success in Modeling.
TLDR
An ideal and repeatable rat brainstem hemorrhage model by injecting autologous blood stereotactically was created, and it mimics the natural onset of brain stem hemorrhage. Expand
Characterization of Axon Damage, Neurological Deficits, and Histopathology in Two Experimental Models of Intracerebral Hemorrhage
TLDR
It is suggested that a modified IC lesioned model was proved to have long lasting neurological deficits, which would provide new insights into the role of whiter matter injury in the mechanism and therapeutic targets of ICH. Expand
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