A mouse knockout library for secreted and transmembrane proteins

  title={A mouse knockout library for secreted and transmembrane proteins},
  author={Tracy Tang and Li Li and Jerry Tang and Yun Li and Wei Yu Lin and Flavius Martin and Deanna Grant and Mark J Solloway and Leon H. Parker and Weilan Ye and W C Melanie Forrest and Nico Ghilardi and Tam{\'a}s Oravecz and Kenneth A. Platt and Dennis S. Rice and Gwenn M. Hansen and Alejandro Abuin and Derek E. Eberhart and Paul J. Godowski and Kathleen H. Holt and Andrew Peterson and Brian P. Zambrowicz and Frederic Sauvage},
  journal={Nature Biotechnology},
Large collections of knockout organisms facilitate the elucidation of gene functions. Here we used retroviral insertion or homologous recombination to disrupt 472 genes encoding secreted and membrane proteins in mice, providing a resource for studying a large fraction of this important class of drug target. The knockout mice were subjected to a systematic phenotypic screen designed to uncover alterations in embryonic development, metabolism, the immune system, the nervous system and the… CONTINUE READING
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