A more specific, liquid-chromatographic method for free cortisol in urine.

@article{Canalis1982AMS,
  title={A more specific, liquid-chromatographic method for free cortisol in urine.},
  author={Ernesto Canalis and Gerald Reardon and A M Caldarella},
  journal={Clinical chemistry},
  year={1982},
  volume={28 12},
  pages={2418-20}
}
Currently used assays for urinary cortisol reportedly overestimate it, owing to cross-reacting substances. We describe here a method for separating and measuring by liquid chromatography cortisol extracted from urine. The method is specific for cortisol and as little as 5 ng per sample can be measured. Mean analytical recovery of added cortisol was 98.8% (SD 6.1%) and the coefficients of variation ranged from 3.1 to 4.7% (within-day) and from 7.1 to 14% (between-day). Mean (and SD) urinary… CONTINUE READING

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