A monophasic solution for isolation of RNA devoid of polymerase chain reaction-detectable genomic DNA contamination.


Commercially available reagents and published protocols are widely used for RNA isolation. However, genomic DNA contamination in isolated RNA is a potential problem. Here we describe a simple, inexpensive method for eliminating genomic DNA contamination beyond the level of PCR-based detection through reduction of the guanidine thiocyanate concentration (1… (More)
DOI: 10.1016/j.ab.2015.02.018