A monoclonal antibody specific for the red-absorbing form of phytochrome

  title={A monoclonal antibody specific for the red-absorbing form of phytochrome},
  author={Mary L. Holdsworth and Garry C. Whitelam},
Characterisation of a new monoclonal antibody (mAb), designated LAS 41, directed against 124-kilodalton (kDa) etiolated-oat (Avena sativa L.) phytochrome, indicates that it recognises an epitope unique to the red-light-absorbing form, Pr. In a solid-phase enzyme-linked immunosorbent assay (ELISA), LAS 41 exhibits a seven- to eight-fold higher affinity for Pr than for the far-red-light-absorbing form of phytochrome, Pfr. In addition, in immunoprecipitation assays LAS 41 effectively precipitates… CONTINUE READING
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