A monoclonal antibody applicable for determination of C-peptide of human proinsulin by RIA.

@article{Hilgert1991AMA,
  title={A monoclonal antibody applicable for determination of C-peptide of human proinsulin by RIA.},
  author={Ivan Hilgert and Pavel {\vS}tolba and Hana Kri{\vs}tofov{\'a} and Irena {\vS}tefanov{\'a} and Bela Bendlova and Michal Lebl and V{\'a}clav Hor̆ejs̆{\'i}},
  journal={Hybridoma},
  year={1991},
  volume={10 3},
  pages={379-86}
}
BALB/c, (BALB/c x B10.A)F1 and (BALB/c x B10)F1 hybrid mice were immunized with C-peptide of human proinsulin. The (BALB/c x B10.A)F1 hybrids were the best responders and yielded 3 hybridomas secreting specific monoclonal antibodies. One of them, C-PEP-01, bound the C-peptide with high affinity (Kas = 1.1 x 10(9) l/mol), cross-reacted fully with human proinsulin but not with insulin, glucagon or somatostatin and apparently recognized the regions of C-peptide comprising amino acid residues 8-13… CONTINUE READING