A monoclonal antibody against a family of nuclear pore proteins (nucleoporins): O-linked N-acetylglucosamine is part of the immunodeterminant.

@article{Park1987AMA,
  title={A monoclonal antibody against a family of nuclear pore proteins (nucleoporins): O-linked N-acetylglucosamine is part of the immunodeterminant.},
  author={Min Kyun Park and MARA D'ONOFRIO and Mark C. Willingham and John A Hanover},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1987},
  volume={84 18},
  pages={6462-6}
}
Using nuclear envelopes from Chinese hamster ovary (CHO) cells as an antigen, a mouse monoclonal antibody (IgM; designated mAb CHON211) that specifically binds to components of the nuclear pore complex (nucleoporins) was isolated. Immunofluorescence localization of the antigen recognized by mAb CHON211 revealed a punctate pattern restricted to the nuclear envelope; this pattern changed dramatically during the cell cycle. When examined by electron microscopy, the antigen was largely restricted… CONTINUE READING

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Using galactosyltransferase and the lectin wheat germ agglutinin as probes , we have previously shown that proteins bearing O - linked N - acetylglucosamine ( GlcNAc ) are restricted to the cytoplasmic and nucleoplasmic faces of the nuclear pore complex .
Using galactosyltransferase and the lectin wheat germ agglutinin as probes , we have previously shown that proteins bearing O - linked N - acetylglucosamine ( GlcNAc ) are restricted to the cytoplasmic and nucleoplasmic faces of the nuclear pore complex .
Nuclear EnvelopeIs associated anatomy of gene productNuclear Pore Complex Proteins
Using nuclear envelopes from Chinese hamster ovary ( CHO ) cells as an antigen , a mouse monoclonal antibody ( IgM ; designated mAb CHON211 ) that specifically binds to components of the nuclear pore complex ( nucleoporins ) was isolated .
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