A monoclonal antibody against a 135-K Golgi membrane protein.

  title={A monoclonal antibody against a 135-K Golgi membrane protein.},
  author={Bernard F. Burke and Gareth Griffiths and Hubert Reggio and Daniel Louvard and Graham Warren},
  journal={The EMBO journal},
  volume={1 12},
A monoclonal antibody ( 53FC3 ) has been produced against a Golgi membrane protein with a mol. wt. of 135 000 which was originally identified using a polyclonal antiserum. Treatment of isolated, intact Golgi vesicles with protease caused a decrease in mol. wt. of 5000-10 000, whereas in the presence of Triton X-100, the protein was completely degraded. This shows that the protein spans the bilayer and that most of its mass is on the luminal side of Golgi membranes. Using two immunoelectron… CONTINUE READING

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