A mollicute (mycoplasma) DNA repair enzyme: purification and characterization of uracil-DNA glycosylase.

@article{Williams1990AM,
  title={A mollicute (mycoplasma) DNA repair enzyme: purification and characterization of uracil-DNA glycosylase.},
  author={M. Williams and J. Pollack},
  journal={Journal of bacteriology},
  year={1990},
  volume={172 6},
  pages={
          2979-85
        }
}
The DNA repair enzyme uracil-DNA glycosylase from Mycoplasma lactucae (831-C4) was purified 1,657-fold by using affinity chromatography and chromatofocusing techniques. The only substrate for the enzyme was DNA that contained uracil residues, and the Km of the enzyme was 1.05 +/- 0.12 microM for dUMP containing DNA. The product of the reaction was uracil, and it acted as a noncompetitive inhibitor of the uracil-DNA glycosylase with a Ki of 5.2 mM. The activity of the enzyme was insensitive to… Expand
Purification and characterization of a cold-adapted uracil-DNA glycosylase from Atlantic cod (Gadus morhua).
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