A molecular method for S-allele identification in apple based on allele-specific PCR

@article{Janssens1995AMM,
  title={A molecular method for S-allele identification in apple based on allele-specific PCR},
  author={Greet A. Janssens and Inge J. W. M. Goderis and Willem F. Broekaert and Wim J. Broothaerts},
  journal={Theoretical and Applied Genetics},
  year={1995},
  volume={91},
  pages={691-698}
}
cDNA sequences corresponding to two self-incompatibility alleles (S-alleles) of the apple cv ‘Golden Delicious’ have previously been described, and now we report the identification of three additional S-allele cDNAs of apple, one of which was isolated from a pistil cDNA library of cv ‘Idared’ and two of which were obtained by reverse transcription-PCR (RT-PCR) on pistil RNA of cv ‘Queen's Cox’. A comparison of the deduced amino acid sequences of these five S-allele cDNAs revealed an average… CONTINUE READING

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