A molecular marker for the identification of Simulium squamosum (Diptera: Simuliidae)

  title={A molecular marker for the identification of Simulium squamosum (Diptera: Simuliidae)},
  author={Rolf Andr{\'e} Mank and M. D. Wilson and Jos{\'e} Miguel Rubio and Rory J Post},
  journal={Annals of Tropical Medicine \& Parasitology},
  pages={197 - 208}
Abstract All except one of the important groups of West African vectors of Onchocerca volvulus that lie within the Simulium damnosum complex can be distinguished from each other using morphological characteristics. The exception is S. squamosum, which overlaps with other species, and this results in significant levels of misidentification. Variation in the untranscribed H3-H4 histone intergenic spacer region of flies of the S. damnosum complex has now been investigated. Although a CAA/CAG… 
A guide to the Simulium damnosum complex (Diptera: Simuliidae) in Nigeria, with a cytotaxonomic key for the identification of the sibling species
The physico–chemical hydrology of the Nigerian breeding sites of the cytospecies does not, however, correspond to that seen elsewhere in West Africa, and it is not clear whether this might be related to differences in the cytoforms.
Confirmation of the species status of the blackfly Simulium galeratum in Britain using molecular taxonomy
A phylogenetic study based upon a region of the mitochondrial cytochrome c oxidase 1 gene (DNA barcoding) produced a tree that delineated the morphotypes into two distinct monophyletic clades, and proposed the re‐instatement of S. galeratum to species status.
Stability and change in the distribution of cytospecies of the Simulium damnosum complex (Diptera: Simuliidae) in southern Ghana from 1971 to 2011
The authors' data had little power to support (or refute) suggestions of a continuing increase in cytospecies distributions in southern Ghana, except in the Asukawkaw river basin, and patterns of distribution were described for the first time.
Onchocerciasis transmission in Ghana: biting and parous rates of host-seeking sibling species of the Simulium damnosum complex
The bionomic differences between sibling species of the S. damnosum complex need to be taken into account when designing entomological monitoring protocols for interventions and parameterising mathematical models for onchocerciasis control and elimination.
Evolution, epidemiology, and population genetics of black flies (Diptera: Simuliidae).
  • P. Adler, R. Cheke, R. Post
  • Biology
    Infection, genetics and evolution : journal of molecular epidemiology and evolutionary genetics in infectious diseases
  • 2010
The subgenus Eusimulium (Diptera: Simuliidae: Simulium) in Britain
Simulium petricolum is reported from southern England, and is shown to be distinct from the other Eusimulium species in terms of mitochondrial DNA sequence (COI DNA barcode), but morphologically and cytotaxonomically indistinguishable from Mediterranean S. peticolum.
A new cytotype of Simulium squamosum from south‐west Cameroon
A new cytotype of Simulium squamosum Enderlein (Diptera: Simuliidae) is described from around Mount Cameroon in south‐west Cameroon on the basis of sex‐chromosome differences on the short arm of chromosome I.
Onchocerciasis Transmission in Ghana: Persistence under Different Control Strategies and the Role of the Simuliid Vectors
Despite repeated ivermectin treatment, evidence of O. volvulus transmission was documented in all seven villages and above the WHO threshold in two, and Vector species influences transmission through biting and parous rates and vector competence, and should be included in transmission models.
Feeding patterns of biting midges of the Culicoides obsoletus and Culicoides pulicaris groups on selected farms in Brandenburg, Germany
Preliminary results reveal that biting midges of the C. pulicaris and C. obsoletus groups can feed on a range of vertebrate hosts but with a distinct preference for cattle even if other livestock are maintained in adjacent areas.
Laser‐assisted microdissection of polytene chromosomes from Diptera for the development of molecular markers
The structural details visible under the light microscope have made polytene chromosomes from Diptera much used in studies of the taxonomy, evolution and genetics of important biological models such


Assessment of rDNA IGS as a molecular marker in the Simulium damnosum complex
Although most sequences from S. squamosum clustered together, a few of them were more similar to those in other cytospecies and could be explained either by hybridization with genetic introgression or by ancestral polymorphism and recent speciation.
Chromosomal identification of eight species of the subgenus Edwardsellum near and including Simulium (Edwardsellum) damnosum Theobald (Deptera: Simuliidae).
: Examinations of the banding pattern of polytene chromsomes from larval salivary glands of the population in west Africa once thought to be a single species, Simulium (Edwardsellum) damnosum,
A pictorial guide to the chromosomal identification of members of the Simulium damnosum Theobald complex in west Africa with particular reference to the Onchocerciasis Control Programme Area.
  • D. Boakye
  • Medicine
    Tropical medicine and parasitology : official organ of Deutsche Tropenmedizinische Gesellschaft and of Deutsche Gesellschaft fur Technische Zusammenarbeit
  • 1993
The diagnostic chromosomal inversions and other micromorphological characters used in the identification of the species found in the OCP area are presented with figures for their recognition, some distributional information is also given.
Molecular identification of onchocerciasis vector sibling species in black flies (Diptera: Simuliidae).
Two molecular assays to identify the sibling species of biting female flies are presented, one of which relies on PCR-mediated amplification of internal transcribed spacers of the nuclear rDNA loci while the second involves immunoblotting of silk proteins which are expressed in both larvae and adult females.
Molecular identification and phylogeny of East African Simulium damnosum s.l. and their relationship with West African species of the complex (Diptera: Simuliidae)
Results suggest that the S. damnosum complex is divided into an East and a West African clade, and S. pandanophilum and the cytoform ‘Kiwira' form an East African subbranch distinct from the ‘Sanje’ group.
DNA probes for the identification of members of the Simulium damnosum complex (Diptera: Simuliidae)
Three cloned repetitive sequences, which show variation in copy number between sibling species, have been isolated and can be used as probes for the dot‐blot identification of larvae, pupae or adults into the three main West African subcomplexes, i.e. damnosum, squamosum and sanctipauli subcom‐plexes.
Morphological identification of simulium sanctipauli and S. yahense in Liberia and comparison of results with those of enzyme electrophoresis.
It was shown that the females of the two species can be separated reliably by the use of external morphological characters and the occasional finding in the same fly of phosphoglucomutase variants typical for each species indicated the possible occurrence of natural hybridization between S. sanctipauli and S. yahense.
Development of a method for the cytological identification of man-biting sibling species within the Simulium damnosum complex.
The method was used to show that the banding pattern is conserved between adult and larval tissues, and consequently it has been possible to demonstrate that within the S. sanctipauli subcomplex both S. soubrense 'B' and S. sanctuaryi bite humans suggests that there is a physiological stage which is optimum for obtaining maximum polyteny for cytotaxonomy.
The distribution of the Simulium damnosum complex in Sierra Leone and its relation to onchocerciasis.
Over most of Sierra Leone onchocerciasis shows a 'forest' epidemiology and is transmitted by'forest' vector cytospecies, however, data from the narrow savanna belt in the extreme north are sparse.