A molecular cytogenetic map of sorghum chromosome 1. Fluorescence in situ hybridization analysis with mapped bacterial artificial chromosomes.

@article{IslamFaridi2002AMC,
  title={A molecular cytogenetic map of sorghum chromosome 1. Fluorescence in situ hybridization analysis with mapped bacterial artificial chromosomes.},
  author={M. Nurul Islam-Faridi and Kevin L. Childs and Patricia E Klein and George M Hodnett and Monica A. Menz and Robert R. Klein and W. L. Rooney and John E Mullet and David M Stelly and Harold J. Price},
  journal={Genetics},
  year={2002},
  volume={161 1},
  pages={345-53}
}
We used structural genomic resources for Sorghum bicolor (L.) Moench to target and develop multiple molecular cytogenetic probes that would provide extensive coverage for a specific chromosome of sorghum. Bacterial artificial chromosome (BAC) clones containing molecular markers mapped across sorghum linkage group A were labeled as probes for fluorescence in situ hybridization (FISH). Signals from single-, dual-, and multiprobe BAC-FISH to spreads of mitotic chromosomes and pachytene bivalents… CONTINUE READING
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