A molecular approach to search for diversity among bacteria in the environment

  title={A molecular approach to search for diversity among bacteria in the environment},
  author={Holger Rheims and Fred A. Rainey and Erko Stackebrandt},
  journal={Journal of Industrial Microbiology},
Molecular 16S rDNA-based techniques were applied to a peat sample from northern Germany in order to investigate the bacterial diversity present and compare the clone sequences with those obtained from similar studies on other terrestrial samples. Genomic DNA was extracted from the peat matrix by a direct lysis procedure. 16S rRNA genes were amplified using PCR primers targeting conserved regions of bacterial 16S rDNA. 16S rDNA fragments were blunt end cloned into a plasmid vector and the… CONTINUE READING
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