A molecular and in silico characterization of Hev b 4, a glycosylated latex allergen.

@article{Malik2008AMA,
  title={A molecular and in silico characterization of Hev b 4, a glycosylated latex allergen.},
  author={Adeel Malik and Siti Arija M Arif and Shandar Ahmad and Elumalai Sunderasan},
  journal={International journal of biological macromolecules},
  year={2008},
  volume={42 2},
  pages={
          185-90
        }
}
Hev b 4 is a heavily glycosylated latex allergen with seven attached N-glycans, comprising of both oligomannose and complex type structures. Treatment with a mixture of N-glycosidase A and N-glycosidase F resulted in lowering Hev b 4 protein on SDS-gel from 53 to 55kDa to circa 40kDa, this being comparable to the 38.53kDa mass predicted by its cDNA. In Western-immunoblots, the enzymatically deglycosylated Hev b 4 showed negligible binding to IgE from latex allergic patients; the results… CONTINUE READING
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