A molecular analysis of ground sloth diet through the last glaciation

  title={A molecular analysis of ground sloth diet through the last glaciation},
  author={Michael Hofreiter and H. N. Poinar and W. Geoffrey Spaulding and Kurt Bauer and P. S. Martin and G{\"o}ran Possnert and Svante P{\"a}{\"a}bo},
  journal={Molecular Ecology},
DNA was extracted from five coprolites, excavated in Gypsum Cave, Nevada and radiocarbon dated to approximately 11 000, 20 000 and 28 500 years bp. All coprolites contained mitochondrial DNA sequences identical to a DNA sequence determined from a bone of the extinct ground sloth Nothrotheriops shastensis. A 157‐bp fragment of the chloroplast gene for the large subunit of the ribulosebisphosphate carboxylase (rbcL) was amplified from the boluses and several hundred clones were sequenced. In… 

Molecular analysis of a 11 700‐year‐old rodent midden from the Atacama Desert, Chile

The more extensive sampling of modern Phyllotis reinforces the suggestion that P. limatus is recently derived from a peripheral isolate, and suggests a much more diverse and humid climate 11 700 years ago.

Mycological evidence of coprophagy from the feces of an Alaskan Late Glacial mammoth

The Ecological implications of a Yakutian mammoth's last meal

Collagen Sequence Analysis of the Extinct Giant Ground Sloths Lestodon and Megatherium

Examination of phylogenetic potential of proteomics-based sequencing through the analysis of collagen extracted from two extinct giant ground sloths, Lestodon and Megatherium highlights that proteomics methods could yield plausible phylogenies that share similarities with other methods, but have the potential to be more useful in fossils beyond the limits of ancient DNA survival.

Integrative analysis of DNA, macroscopic remains and stable isotopes of dog coprolites to reconstruct community diet

An integrative analysis of dog coprolites is presented, combining macroscopic analyses, stable isotope measurements, and DNA shotgun sequencing to examine diet and health status, finding that the Late Woodland dogs consumed a variety of fish as well as bird and plant taxa, possibly including maize, and also harbored intestinal parasites and pathogenic bacteria.

Diet analysis of Leopoldamys neilli, a cave-dwelling rodent in Southeast Asia, using Next-Generation Sequencing from feces

Examining the plant composition of the diet of L. neilli at the level of order and family using DNA for molecular identification and to compare it with two other forest-dwelling Leopoldamys species, L. herberti and L. sabanus, identified seventeen orders and twenty-one plant families corresponding to thirty-three putative species.

A molecular analysis of dietary diversity for three archaic Native Americans

  • H. PoinarM. Kuch S. Pääbo
  • Biology
    Proceedings of the National Academy of Sciences of the United States of America
  • 2001
Human paleofecal remains represent a source of ancient DNA that significantly complements and may in some cases be superior to that from skeletal tissue.

Plant DNA sequences from feces: potential means for assessing diets of wild primates

With further optimization, this method could provide a basic evaluation of minimum primate dietary diversity even when knowledge of local flora is limited and may find application in studies characterizing the diets of poorly‐known, unhabituated primate species or assaying consumer–resource relationships in an ecosystem.

Paleo‐metagenomics of North American fossil packrat middens: Past biodiversity revealed by ancient DNA

The use of shotgun metagenomics is explored to study the aDNA obtained from packrat middens up to 32,000 C14 years old to provide highly detailed characterizations of past communities of plants, animals, bacteria, and fungi present as trace DNA fossils.



Molecular coproscopy: dung and diet of the extinct ground sloth Nothrotheriops shastensis.

DNA from excrements can be amplified by means of the polymerase chain reaction. However, this has not been possible with ancient feces. Cross-links between reducing sugars and amino groups were shown

Molecular phylogeny of the extinct ground sloth Mylodon darwinii.

Phylogenetic analyses using homologous sequences from all extant edentate groups suggest that Mylodon darwinii was more closely related to the two- toed than the three-toed sloths and, thus, that an arboreal life-style has evolved at least twice among sloths.

DNA extraction from Pleistocene bones by a silica-based purification method.

The following method, which is a modification of a protocol published by Boom et a/.

Development of Vegetation and Climate in the Southwestern United States

Plant macrofossils in ancient packrat middens document the presence of woodland communities in most of the present Chihuahuan, Sonoran, and Mohave deserts in the southwestern United States during the

The retrieval of ancient human DNA sequences.

These results show that more experimental work than is often applied is necessary to ensure that DNA sequences amplified from ancient human remains are authentic and quantitation of the numbers of amplifiable molecules is a useful tool to determine the role of contaminating contemporary molecules and PCR errors in amplifications from ancient DNA.

DNA damage and DNA sequence retrieval from ancient tissues.

Gas chromatography/mass spectrometry (GC/MS) was used to determine the amounts of eight oxidative base modifications in DNA extracted from 11 specimens of bones and soft tissues, ranging in age from

Late Holocene Vegetation Changes in Greenwater Valley, Mojave Desert, California

  • K. ColeR. Webb
  • Environmental Science, Geography
    Quaternary Research
  • 1985

Death of American Ground Sloths

Dates from other caves in the arid Southwest indicate that the Shasta ground sloth disappeared at very soon after the time of Clovis big game hunters, in accord with the model of explosive overkill.

Fossil spring deposits in the southern Great Basin and their implications for changes in water-table levels near Yucca Mountain, Nevada, during Quaternary time

Fossil spring deposits are common in the southern Great Basin, and their distribution provides important constraints on the hydrologic response of the regional water table to climate change. This