A modified ultraviolet spectrophotometric method for the determination of theophylline in serum in the presence of barbiturates.

@article{Vasiliades1976AMU,
  title={A modified ultraviolet spectrophotometric method for the determination of theophylline in serum in the presence of barbiturates.},
  author={John Vasiliades and Terence Turner},
  journal={Clinica chimica acta; international journal of clinical chemistry},
  year={1976},
  volume={69 3},
  pages={
          491-5
        }
}
Ultraviolet spectrophotometry is the most commonly used technique for the determination of theophylline levels for therapeutic monitoring. Common interferences in most methods are barbiturates and xanthines. A modified method which eliminates interferences from barbiturates and most xanthines is presented. Theophylline is extracted from serum with chloroform/isopropanol at pH 7.4 back extracted into dilute hydrochloric acid and then the solution is made alkaline with sodium hydroxide… CONTINUE READING