A modified protocol for rapid DNA isolation from plant tissues using cetyltrimethylammonium bromide

@article{Allen2006AMP,
  title={A modified protocol for rapid DNA isolation from plant tissues using cetyltrimethylammonium bromide},
  author={Gregory C. Allen and Miguel A Flores-Vergara and S Krasynanski and Suresh N. Kumar and William F. Thompson},
  journal={Nature Protocols},
  year={2006},
  volume={1},
  pages={2320-2325}
}
We describe a modification of the DNA extraction method, in which cetyltrimethylammonium bromide (CTAB) is used to extract nucleic acids from plant tissues. In contrast to the original method, the modified CTAB procedure is faster, omits the selective precipitation and CsCl gradient steps, uses less expensive and toxic reagents, requires only inexpensive laboratory equipment and is more readily adapted to high-throughput DNA extraction. This protocol yields approximately 5–30 μg of total DNA… CONTINUE READING
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