A modified procedure for rapid labelling of low concentrations of bioactive proteins with indium-111.

Abstract

We describe the conjugation of DTPA to 100-500 micrograms of protein in concentrations of 0.6-1.0 mg/mL utilizing the mixed anhydride method. Free DTPA is removed by minicolumn gel filtration and centrifugation with minimal protein dilution. Radiolabelling at any selected pH can be achieved easily by diluting the protein in the desired buffer. The… (More)

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Cite this paper

@article{Zoghbi1985AMP, title={A modified procedure for rapid labelling of low concentrations of bioactive proteins with indium-111.}, author={Sami S. Zoghbi and Ronald D. Neumann and Alexander Gottschalk}, journal={International journal of nuclear medicine and biology}, year={1985}, volume={12 3}, pages={159-66} }