A modified procedure for fast purification of T7 RNA polymerase.

@article{Li1999AMP,
  title={A modified procedure for fast purification of T7 RNA polymerase.},
  author={Yuan-shen Li and En Duo Wang and Yi Li Wang},
  journal={Protein expression and purification},
  year={1999},
  volume={16 2},
  pages={
          355-8
        }
}
The in vitro T7 transcription system allows one to synthesize biochemical amounts of RNA molecules functionally equivalent or similar to those transcripts normally existing at extremely low levels in vivo. In this study we described a modified method for efficient large-scale preparation of pure T7 RNA polymerase free of RNase activity from the recombinant Escherichia coli strain BL21/pAR1219 (4). The procedure, which used preparative column chromatography on DEAE-Sepharose CL-6B and Blue 3GA… CONTINUE READING
BETA

From This Paper

Topics from this paper.

Citations

Publications citing this paper.
SHOWING 1-10 OF 18 CITATIONS

Similar Papers

Loading similar papers…