A modified host-cell reactivation assay to measure repair of alkylating DNA damage for assessing risk of lung adenocarcinoma.

@article{Wang2007AMH,
  title={A modified host-cell reactivation assay to measure repair of alkylating DNA damage for assessing risk of lung adenocarcinoma.},
  author={Luo Yi Wang and Qingyi Wei and Qiuling Shi and Zhaosheng Guo and Yawei Qiao and Margaret R. Spitz},
  journal={Carcinogenesis},
  year={2007},
  volume={28 7},
  pages={1430-6}
}
The nicotine-derived nitrosamine 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) induces lung adenocarcinoma through formation of DNA adducts. Our previous research on susceptibility to tobacco-induced carcinogenesis focused on benzo[a]pyrene diol epoxide (BPDE) as the in vitro mutagen for phenotype measurements of DNA repair capacity (DRC) in mammalian cells. Here, we present a modified host-cell reactivation (HCR) assay to measure lymphocytic DRC for alkylating DNA damage as is induced… CONTINUE READING