A modified colorimetric assay of macrophage activation for intracellular cytotoxicity against Leishmania parasites.

@article{Kiderlen1990AMC,
  title={A modified colorimetric assay of macrophage activation for intracellular cytotoxicity against Leishmania parasites.},
  author={Albrecht Ferdinand Kiderlen and Paul M. Kaye},
  journal={Journal of immunological methods},
  year={1990},
  volume={127 1},
  pages={11-8}
}
An in vitro method is described which colorimetrically assesses the activation of macrophages for intracellular cytotoxicity against the obligate intracellular parasite Leishmania donovani. The assay system uses a highly purified macrophage population derived from 10-day murine bone marrow cultures. These were infected in vitro as a suspension culture with viable L. donovani amastigotes and then exposed to activating agents. After 48 h the intracellular parasites were released by SDS lysis of… CONTINUE READING