A modified bacterial one-hybrid system yields improved quantitative models of transcription factor specificity

Abstract

We examine the use of high-throughput sequencing on binding sites recovered using a bacterial one-hybrid (B1H) system and find that improved models of transcription factor (TF) binding specificity can be obtained compared to standard methods of sequencing a small subset of the selected clones. We can obtain even more accurate binding models using a modified… (More)
DOI: 10.1093/nar/gkr239

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