A modified PCR-RFLP genotyping method demonstrates the presence of the HPA-4b platelet alloantigen in a North American Indian population

@article{Reiner1997AMP,
  title={A modified PCR-RFLP genotyping method demonstrates the presence of the HPA-4b platelet alloantigen in a North American Indian population},
  author={Anton Reiner and Gayle Teramura},
  journal={Immunohematology},
  year={1997},
  volume={13},
  pages={37 - 43}
}
The diallelic HPA-4 (Pen/Yuk) platelet alloantigen system is polymorphic in Asian populations and accounts for the majority of cases of neonatal alloimmune thrombocytopenia in Japan. At the molecular level, the HPA-4a/4b dimorphism is associated with an arginine/ glutamine substitution at amino acid 143 of the gene encoding platelet glycoprotein IIIa. Unlike the five other major diallelic human platelet antigen systems (HPA-1, -2, -3, -5, and -6), the nucleotide substitution corresponding to… 
2 Citations

Figures and Tables from this paper

Analysis of platelet glycoprotein Ia (alpha2 integrin) allele frequencies in three North American populations reveals genetic association between nucleotide 807C/T and amino acid 505 Glu/Lys (HPA-5) dimorphisms.
TLDR
The results indicate a genetic relationship between the 807C/T and Glu/Lys505 dimorphisms that leads to an evolutionary model of GPIa isoforms.
Physiological and ethnogenetic risk factors for cardiovascular thrombosis
An analysis of publications on the polymorphism of enzymes, receptors, and other systems of lipid and lipoprotein metabolism critical for the formation of vascular lipoprotein plaques and thrombi

References

SHOWING 1-10 OF 37 REFERENCES
Genomic RFLP typing of human platelet alloantigens Zw(PlA), Ko, Bak and Br (HPA‐1, 2, 3, 5)
TLDR
Serologically obtained data (MAIPA and platelet agglutination) were compared with results from analysis of restriction fragment length polymorphisms (RFLP) and Discordances were found in the HPA‐2 and 3 systems and can be ascribed to false typing results in both the serological and genomic methods.
Rapid genotyping of the five major platelet alloantigens by reverse dot-blot hybridization.
TLDR
The reverse dot-blot (RDB) technique was used to successfully genotype women and family members with neonatal alloimmune thrombocytopenia and with posttransfusion purpura and to prenatally genotype the amniocytes from a fetus at risk for thromBocy topenia.
Genotype Frequencies of the Human Platelet Antigen, Ca/Tu, in Japanese, Determined by a PCR‐RFLP Method
TLDR
The present study showed that the frequency of Ca/Tua individuals in the Japanese was approximately 7‐fold higher than in the Finnish population, and attention must be given to the involvement of the Ca/Tu alloantigen in neonatal alloimmune thrombocytopenia and the refractoriness of platelet transfusion.
Sequence Variation of Human Platelet Membrane Glycoprotein IIIa Associated with the Yuka/Yukb Alloantigen System.
TLDR
Slot blotting analysis using sequence-specific oligonucleotide probes demonstrated that this Arg/Gln polymorphism is associated with the Yuk alloantigen system.
Simultaneous DNA Typing of Human Platelet Antigens 2, 3 and 4 by an Allele‐Specific PCR Method
We developed an allele‐specific polymerase chain reaction (ASPCR) method using originally designed primers to determine the genotype of the human platelet antigens (HPAs) 2, 3 and 4 in parallel. The
An amino acid polymorphism within the RGD binding domain of platelet membrane glycoprotein IIIa is responsible for the formation of the Pena/Penb alloantigen system.
TLDR
It appears that amino acid 143 of GPIIIa is not only associated with Pen phenotype, but specifically controls the formation and expression of the Pen alloantigenic determinants.
DNA‐Based Typing of Human Platelet Antigen Systems by Polymerase Chain Reaction‐Single‐Strand Conformation Polymorphism Method
TLDR
The results indicate that PCR‐SSCP is a simple and sensitive method for determining HPA genotypes and identifying unknown polymorphisms.
Non‐radioactive SSCP for genotyping human platelet alloantigens
TLDR
A rapid method of genotyping which does not use restriction enzymes and is less prone to misinterpretation is reported, which is non‐radioactive PCR‐SSCP (single strand conformation polymorphism), which is illustrated for two different HPA system.
Molecular characterization of glucose-6-phosphate dehydrogenase (G6PD) deficiency by natural and amplification created restriction sites: five mutations account for most G6PD deficiency cases in Taiwan.
TLDR
It is shown that G to T change at nt 1376 is the most common mutation, and research indicates that nt 493 mutation is a frequent mutation among Chinese in Taiwan.
...
...