A modified Coomassie blue staining of proteins in polyacrylamide gels with Bismark brown R.

@article{Choi1996AMC,
  title={A modified Coomassie blue staining of proteins in polyacrylamide gels with Bismark brown R.},
  author={Jung-Kap Choi and So Hee Yoon and Hi Young Hong and Dai Ki Choi and Gun Suk Yoo},
  journal={Analytical biochemistry},
  year={1996},
  volume={236 1},
  pages={82-4}
}
A rapid and sensitive protein staining method employing a mixed dye technique has been developed. Solutions of Coomassie brilliant blue R-250 (CB, 0.2%) and Bismark brown R (BBR, 0.05%) were mixed in the volume ratio of 1:0.75 for staining (final molar ratio, 4.5:1). In this staining, BBR inhibits the binding of CB to gel matrix by forming ion-pairing complex with excess CB; therefore, it reduces staining/destaining times and also enhances the staining effect of CB on protein band. As a result… CONTINUE READING

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