A modification of tooth germ cultivation in vitro and in ovo

@article{Peterka1991AMO,
  title={A modification of tooth germ cultivation in vitro and in ovo},
  author={Miroslav Peterka and V{\'a}clav Mandys and Renata Peterkova},
  journal={Cytotechnology},
  year={1991},
  volume={7},
  pages={49-53}
}
Mandibular molar anlages excised from 17-day mouse foetuses were cultured in vitro or in ovo (on the chorioallantoic membrane). In both cases, the explants were underlain either with a Millipore filter or with a piece of fibrin foam. Tooth germs were harvested after 7 days of cultivation and processed histologically. Spatial arrangement was highly preserved in the tooth germs cultured in vitro on fibrin foam. In vitro cultures on Millipore filters revealed significant flattening of tooth germs… CONTINUE READING