- G. I. Abelev, Z. A. Avenixova
- V. Engel'gardt et. aL. Doklady Akad. Nauk SSSR…
Antigens and antisera are placed in depression side the angles of a squere. Each antigen is located diagonally opposite the homologous serum. The antigens specific for each system give precipitation lines crossing at the center of the square at right angles to each other, their ends resting against the depressions of the heterologous system. The antigens common for the systems under comparison produce lines passing between the reservoirs of both antigens and sera. This method facilitates the detection of qualitatively different antigens in the preparations under comparison and permits the analysis of two complete antigen-antiserum systems.