A model virus-cell system to study the persistence of African swine fever virus

  title={A model virus-cell system to study the persistence of African swine fever virus},
  author={Carmen Gil-Fern{\'a}ndez and Dolores Garc{\'i}a-Villal{\'o}n},
  journal={Archives of Virology},
SummaryThe persistence of African swine fever virus (ASFV) on Vero cells was induced by using 5-iodo-2′-deoxyuridine (IDU). After the persistence was established, several cycles of decreasing virus production were observed with intervals in which no virus could be detected. These latency-like periods could last from 15 to 25 days. After three and a half months the cells appeared to be “cured” and no virus was detected during almost three years. These “cured” cells (Vero-L) were more resistant… 

Interferon cures cells lytically and persistently infected with African swine fever virus in vitro

The finding that continuous treatment with IFN-α cures Vero cells from lytic and persistent infections with ASF virus is found, suggesting a potential application of IFN for the treatment of animals carrying the virus.



Establishment of a Vero cell line persistently infected with African swine fever virus

Virus production was low when the Vero-P cells were growing logarithmically and increased considerably in confluent cultures when lysis appeared in a fraction of the cell population, and virus titers were comparable to those obtained in Vero cells acutely infected with African swine fever virus.

Viral Persistence: Evolution of Viral Populations

This chapter will concentrate on another aspect of persistent viral infection, namely, the evolution of virus populations in infections initiated by virulent viruses in cell culture model systems or in immunologically competent animals.

Effects of Ribavirin on BHK-21 Cells Acutely or Persistently Infected With Mumps Virus

Ribavirin had marked antiviral activity against both the acute and persistent infections as determined by an inhibition of hemadsorption plaque formation, decreased immunofluorescence, and a reduction in the release of infectious virus.

Ribavirin cures cells of a persistent infection with foot-and-mouth disease virus in vitro

In treated cells no viral RNA or proteins could be detected by dot-blot hybridization to cDNA probes, virus and RNA infectivity assays, or immunofluorescence, and a potential application of the drug for the treatment of animals carrying the virus is suggested.

Persistent Viral Infections as Models for Research in Virus Chemotherapy

Titration of African swine fever (ASF) virus.

A plaque assay for ASF virus adapted to grow in VERO cells gives a titre similar to that obtained using the haemadsorption microtest, and in both the micromethod and the plaque assay infection may be produced by a single infective particle.