A model of the sodium dependence of dopamine uptake in rat striatal synaptosomes

@article{Wheeler2005AMO,
  title={A model of the sodium dependence of dopamine uptake in rat striatal synaptosomes},
  author={D. D. Wheeler and A. M. Edwards and B. M. Chapman and Jerome G. Ondo},
  journal={Neurochemical Research},
  year={2005},
  volume={18},
  pages={927-936}
}
Initial velocity of uptake of dopamine (DA) has been measured in rat striatal synaptosomes as a function of both [DA] and [Na]. Carrier mediated uptake is totally dependent on external sodium. The data were fitted to a rapid equilibrium model which has been found in previous studies to fit, with appropriate simplification, uptake data for glutamate, GABA, and choline in several brain regions under varying conditions. This model also gives a good fit to the dopamine data. The minimal best fit… 

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References

SHOWING 1-10 OF 30 REFERENCES

Are there both low-and high-affinity glutamate transporters in rat cortical synaptosomes?

TLDR
A rather comprehensive model of glutamate function can be used to describe function in terms of mathematical equations and to make predictions from these equations, which relates velocity of uptake and the kinetic parameters to sodium and substrate concentration, velocity to membrane potential, distribution ratio to the electrochemical potential, and release to time, compartment sizes, and exchange constants.

Kinetics and Block of Dopamine Uptake in Synaptosomes from Rat Caudate Nucleus

TLDR
The data are consistent with a carrier‐mediated DA transport mechanism in which each DA molecule entering the nerve terminal via the carrier is accompanied by two or more Na ions and one Cl ion in a rheogenic process carrying one or more net positive charges into the cell.

A MODEL OF HIGH AFFINITY CHOLINE TRANSPORT IN RAT CORTICAL SYNAPTOSOMES

TLDR
Initial velocity of choline uptake by cortical synaptosomes from the Long‐Evans rat has been measured as a function of both choline and sodium concentration and several possible models which characterize the participation of sodium in the transport process were identified.

The effect of membrane potential on initial velocity of GABA uptake and steady state distribution ratio in rat cortical synaptosomes

TLDR
Initial velocity of synaptosomal GABA uptake and steady state distribution ratio have been measured and are consistent with this assumption if it is also assumed that an anion is co-transported with each GABA molecule.

Kinetics ofd-aspartic acid release from rat cortical synaptosomes

TLDR
There are some quantitative differences in the estimates of the compartment sizes and exchange constants, which are obtained by optimizing the fit of the data to the equation for each model, although most of these quantitative differences appear to be minor.

SODIUM‐DEPENDENT, HIGH AFFINITY CHOLINE UPTAKE

TLDR
This review will focus on choline transport into cholinergic neurons, especially by means of the sodium-dependent, high affinity choline uptake system (SDHACU) which appears to be involved in ACh synthesis and its regulation.

Effects of veratridine and cocaine on the kinetics of synaptosomal dopamine release.

Following preloading of striatal synaptosomes with 3H-dopamine (DA), the kinetics of release have been followed for a 60-min incubation period. DA appears to be totally releasable under both

HIGH AFFINITY UPTAKE OF TRANSMITTERS: STUDIES ON THE UPTAKE OF l‐ASPARTATE, GABA, l‐GLUTAMATE AND GLYCINE IN CAT SPINAL CORD

TLDR
Subcellular fractionation studies indicated that osmotically‐sensitive particles of equilibrium density equivalent to that of 1.0 m‐sucrose were at least in part responsible for the uptake of these amino acids.