A minichaperone-based fusion system for producing insoluble proteins in soluble stable forms.

@article{Sharapova2016AMF,
  title={A minichaperone-based fusion system for producing insoluble proteins in soluble stable forms.},
  author={Olga A Sharapova and Maria S Yurkova and Alexey N Fedorov},
  journal={Protein engineering, design & selection : PEDS},
  year={2016},
  volume={29 2},
  pages={57-64}
}
We have developed a fusion system for reliable production of insoluble hydrophobic proteins in soluble stable forms. A carrier is thermophilic minichaperone, GroEL apical domain (GrAD), a 15 kDa monomer able to bind diverse protein substrates. The Met-less variant of GrAD has been made for further convenient use of Met-specific CNBr chemical cleavage, if desired. The Met-less GrAD retained stability and solubility of the original protein. Target polypeptides can be fused to either C-terminus or… CONTINUE READING