A mini-Mu transposon-based method for multiple DNA fragment integration into bacterial genomes

@article{Wei2010AMT,
  title={A mini-Mu transposon-based method for multiple DNA fragment integration into bacterial genomes},
  author={Xiao-Xing Wei and Zhen-Yu Shi and Zheng-jun Li and Lei Cai and Qiong Wu and Guo-Qiang Chen},
  journal={Applied Microbiology and Biotechnology},
  year={2010},
  volume={87},
  pages={1533-1541}
}
A method for construction of bacterial strains with multiple DNA inserted into their chromosomes has been developed based on the mini-Mu transposon and FLP/FRT recombination. Exogenous DNA can be integrated by Mu transposition with an FRT cassette containing selection marker and conditional replicative origin (R6Kγori). Subsequently, with the introduction of a helper plasmid bearing gene of FLP recombinase, drug-resistant selection marker is excised from the chromosome. Cells cured of the… CONTINUE READING
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Molecular cloning: a laboratory manual, 3rd edn. Cold Spring Harbor Laboratory Press, Cold Spring Harbor

  • J Sambrook, DW Russell
  • 2001
Highly Influential
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