A mild, rapid, and efficient method of lipid extraction for use in determining vitamin E/lipid ratios

Abstract

A new, general method for lipid extraction and measurement of vitamin E/total lipid ratios in tissue and cell samples has been developed. The new extraction procedure use a combination of sodium dodecylsulfate, ethanol andn-heptane, and is mild, clean, convenient, efficient and rapid (≤5 min). The efficiency of the new method has been confirmed for human plasma, red blood cells and rat liver homogenate by the comparison of the yields of vitamin E, O-acyl lipid and cholesterol with the yields obtained following conventional extraction procedures. Extraction efficiency also has been confirmed for multilamenllar vesicles composed of known quantities of vitamin E, egg lecithin and cholesterol.

DOI: 10.1007/BF02534359

7 Figures and Tables

Statistics

0200400600'92'95'98'01'04'07'10'13'16
Citations per Year

2,885 Citations

Semantic Scholar estimates that this publication has 2,885 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Burton1985AMR, title={A mild, rapid, and efficient method of lipid extraction for use in determining vitamin E/lipid ratios}, author={Graham W . Burton and Ann R. Webb and Keith U . Ingold}, journal={Lipids}, year={1985}, volume={20}, pages={29-39} }