A mild procedure for separating polypeptide chains prior to immunoprecipitation and western blotting analysis.

Abstract

Conventional cleavage of linked polypeptide chains by heating in SDS can so alter molecular structure as to interfere with antibody binding, on which both immunoprecipitation and 'western blotting' depend. As an alternative, gentle treatment with acid at room temperature or at 0 degrees C was effective in separating the alpha and beta chains of human MHC Class II glycoprotein dimers and proved superior in terms of preservation of at least one labile epitope on the beta chain.

Cite this paper

@article{Cohen1984AMP, title={A mild procedure for separating polypeptide chains prior to immunoprecipitation and western blotting analysis.}, author={Baruch Cohen and Michael A. Moxley and David N. Crichton and David L Deane and C. Michael Steel}, journal={Journal of immunological methods}, year={1984}, volume={75 1}, pages={99-105} }