A microwave-based method for nucleic acid isolation from environmental samples.

@article{Orsini2001AMM,
  title={A microwave-based method for nucleic acid isolation from environmental samples.},
  author={Maria Rosa Orsini and Vincenzo Romano-Spica},
  journal={Letters in applied microbiology},
  year={2001},
  volume={33 1},
  pages={17-20}
}
AIMS A simple and rapid method was described for DNA isolation directly from activated sludge or other environmental sources, including soil and sediments. METHODS AND RESULTS The present method is based on microwave thermal shock and provides DNA suitable for further analysis. It is also effective for RNA extraction. CONCLUSION The protocol is effective, easy, fast and does not require the use of expensive equipment or reagents. SIGNIFICANCE AND IMPACT OF THE STUDY The described method… CONTINUE READING