A microtiter-plate assay of nitric oxide synthase activity.

  title={A microtiter-plate assay of nitric oxide synthase activity.},
  author={John Dawson and Richard Graham Knowles},
  journal={Molecular biotechnology},
  volume={12 3},
We describe here a microtiter-plate assay for measuring nitric oxide synthase (NOS) activity by utilizing the spectral shift in optical absorbence between the wavelengths 405 and 420 nm on conversion of oxyhemoglobin to methemoglobin by nitric oxide (NO). This is a high-throughput assay permitting 96 or 384 simultaneous kinetic measurements and is ideal for the study of NOS inhibitors and their time dependence. It is also possible to measure enzyme rates under different conditions… CONTINUE READING